Skip to main content
2024 ASAS Midwest Section Meeting Main banner
Back

Category: NONRUMINANT NUTRITION

Poster Session II

PSII-19 - Fecal microbiota composition of weaned piglets under mixed management and poor housing conditions fed diets increased in threonine, methionine and tryptophan

Monday, March 11, 2024
10:00 AM – 10:45 AM CST
Location: Grand Terrace

    Presenting Author(s)

  • Danilo Alves Marçal

    Postdoc
    São Paulo State University (UNESP)
    Jaboticabal, Sao Paulo, Brazil

    • Author(s)

  • AB

    Antonio Diego Brandão Brandao Melo

    School of Agricultural and Veterinarian Sciences, Universidade Estadual Paulista (Unesp), São Paulo, Jaboticabal, Brazil, 14884-900.
    Jaboticabal, Sao Paulo, Brazil

  • JG

    Joseane Penteado Rosa Gonçalves

    São Paulo State University
    Jaboticabal, Sao Paulo, Brazil

  • QY

    Qinnan Yang

    University of Nebraska–Lincoln
    Lincoln, Nebraska, United States

  • Md

    Marllon José Karpeggiane de Oliveira

    School of Agricultural and Veterinarian Sciences, Universidade Estadual Paulista (Unesp), São Paulo, Jaboticabal, Brazil, 14884-900.
    Jaboticabal, Sao Paulo, Brazil

  • PR

    Pedro Righetti Arnaut

    PhD Student
    AGRIFOOD/UNESP
    Jaboticabal, Sao Paulo, Brazil

  • MO

    Manoela Trevizan Ortiz

    São Paulo State University
    Jaboticabal, Sao Paulo, Brazil

  • IF

    Ismael França

    School of Agricultural and Veterinarian Sciences, Universidade Estadual Paulista (Unesp), São Paulo, Jaboticabal, Brazil, 14884-900.
    Jaboticabal, Sao Paulo, Brazil

  • Graziela Alves da Cunha Valini, MSc

    PhD Student
    School of Agricultural and Veterinarian Sciences, Universidade Estadual Paulista (Unesp), São Paulo, Jaboticabal, Brazil, 14884-900.
    Jaboticabal, Sao Paulo, Brazil

  • NK

    Nate Korth

    University of Nebraska, Lincoln, NE, USA. Nebraska Food for Health Center, University of Nebraska, Lincoln, NE, USA.
    Lincoln, Nebraska, United States

  • NP

    Natasha Pavlovikj

    University of Nebraska–Lincoln
    Lincoln, Nebraska, United States

  • PC

    Paulo Henrique Reis Furtado Campo

    Federal University of Viçosa
    Viçosa, Minas Gerais, Brazil

  • HB

    Henrique Gastmann Brand

    Technical Service Manager
    Evonik
    São Paulo, Sao Paulo, Brazil

  • John K. Htoo

    Director Technical Support Swine
    Evonik Operations GmbH
    Hanau, Hessen, Germany

  • JG

    João Carlos Gomes-Neto

    University of Nebraska, Lincoln, NE, USA. Nebraska Food for Health Center, University of Nebraska, Lincoln, NE, USA.
    Lincoln, Nebraska, United States

  • AB

    Andrew Benson

    University of Nebraska–Lincoln
    Lincoln, Nebraska, United States

  • LH

    Luciano Hauschild, Sr.

    Professor
    School of Agricultural and Veterinarian Sciences, Universidade Estadual Paulista (Unesp), São Paulo, Jaboticabal, Brazil, 14884-900.
    Jaboticabal, Sao Paulo, Brazil

Abstract: This study evaluated the effects of increased dietary Thr, Met and Trp supplementation on fecal microbiota composition of weaned piglets raised under contrasting sanitary conditions ([GOOD SC] or under mixed management and poor housing conditions [MM+POOR SC]) during the nursery phase. A total of 144 entire male piglets (6.33 ± 0.91 kg) from a high sanitary status (HSS) farm were randomly housed following a 2×2 factorial arrangement regarding BW blocks. Factorial arrangement was composed of two contrasting SC (GOOD or MM+POOR) and two diets (control, with nutritional requirements according to NRC (2012); or supplemented with Thr:Lys, Met+Cys:Lys and Trp:Lys 20% above the control). In total, 12 pens with three piglets each were used for treatment. On day -2, the 144 HSS piglets were allocated to one of two facilities under contrasting SCs. Also on day -2, to mimic a mixed management in the MM+POOR SC, 48 entire male piglets, from a commercial farm with low sanitary status (LSS), were randomly allocated by two piglets per pen into the same pens where the three HSS piglets were. On day 0, the two LSS piglets were removed from the HSS pens and allocated as sentinels into the remaining pens (four/pen) located between pens with HSS piglets. The MM+POOR SC had a weekly cleaning routine only to remove the manure located under the slatted floor, while in the GOOD SC, a daily cleaning routine and a high level of biosecurity protocol were adopted. One fecal sample per pen was collected on days 0, 21 and 42. The V4 region of the bacterial 16S rRNA gene was amplified from each fecal sample to evaluate ecological changes into bacterial communities and taxonomy. Mean relative abundance for each family/genus/species (taxon) across groups were depicted as a heatmap using the log2 transformed relative abundance of the most dominant taxa based on a 2% cutoff and network analysis of clusters of co-occurrences of the most relevant taxa. Overall, fecal microbiome analyses revealed no differences (P >0.05) in bacterial community composition and individual taxon abundances at the bacterial genus level across treatments over time. The alpha- and beta-diversity analysis showed no diet or SC differences (P >0.05). Additional community structure analysis based on co-occurrence network mapping revealed the absence of major temporal changes in architecture or keystone nodes (taxa) for HSS animals housed in either GOOD or MM+POOR SCs, despite the AA+ supplementation. The analysis revealed no specific taxon or taxa that were preferentially enriched or diminished over time (P >0.05). According to our results, the microbial fecal composition of weaned piglets under GOOD or MM+POOR SCs measured using 16S rRNA sequencing analysis was stable including the major central taxa with no Thr, Met and Trp supplementation effect.